Fig. 7
From: Single-cell sequencing reveals PTX3 involvement in ovarian cancer metastasis
Correlation of PTX3 expression with TFs and microenvironmental changes. (A) Heatmap showing the distribution of 6 identified TF modules in tumor cells. (B) Box plots showing the levels of TFs in tumor cells with high or low PTX3 expression within each TF module. (C) Rank for regulons in tumor cells with high or low PTX3 expression based on regulon specificity score (RSS). (D) Representative images of the morphology of OC cell lines (OVCAR3) in adherent culture and suspension culture systems. Scale bar, 100 μm. (E) PTX3 protein expression in OC cells under adherent and suspension culture conditions (left). Quantitative analysis of PTX3 (right). (F) Western blot detected the expression of PTX3 in OC cells after separate culture and co-culture with peritoneal mesothelial cells (HMrSV5). Quantitative analysis of PTX3 (right). Data are presented as mean ± SD; * P < 0.05, ** P < 0.01