Fig. 5

Photomicrograph of H&E-stained ovarian sections of albino rats (a, b) in the control group a. Normal ovarian follicles at different developmental stages (red arrows), normal stromal cells (yellow arrowhead), and a well-developed corpus luteum (yellow star) X100; b Normal ovarian follicles (red arrows) with normal granulosa cells having vesicular nuclei (black arrow) and a typical oocyte (black star) surrounded by a well-developed zona pellucida (black arrowhead). Normal interstitial cells were also observed (yellow arrowhead) at X400; c,d in the CS-SeNP-treated group; c normal ovarian follicles at different stages of development (red arrows), normal stroma (arrowhead), and a well-developed corpus luteum (yellow star) at X100 d. showing mature follicles with normal oocyte and typical zona pellucida (black star), normal granulosa cells with vesicular nuclei (yellow arrow), and antrum (double-headed black arrow) at X400; e–g Ag-NPs exposed group e. showing hemorrhage with dilated congested blood vessels (black arrows), atretic deformed follicles (green arrows), hyalinization (H), and focal areas of necrosis (black arrowheads) at X100; f degenerated follicle with dissociated hyperplastic granulosa cells having pyknotic nuclei (yellow arrow), degenerated oocyte with detached zona pellucida (yellow arrowhead), and fibrosis (yellow star) at X400. g Fatty degeneration of lutein cells (red arrow) and extravasated blood (green arrowheads) X400. h&i Ag-NPs plus CS-SeNP coadministration group h. recovery with small areas of fatty degeneration (black arrowhead) and a clear reduction in congestion (black arrow) X100. i Nearly normal ovarian follicle with a normal oocyte surrounded by an intact zona pellucida (yellow arrowhead); most of the granulosa cells exhibited vesicular nuclei (yellow arrow), while only a few cells exhibited dark nuclei (red arrow). Some detached granulosa cells were observed in the liquor folliculi (black arrow) X400