Fig. 3

Characterization of the isolated extracellular vesicles (EVs) from human uterine luminal fluid. A Representative image of transmission electron microscopy (TEM) images showing morphology of isolated EVs purified from human uterine luminal fluid. B Nanoparticle tracking analysis by ZetaView PMX120 indicated that the mean diameter of the EVs was 93.6 nm. C Western blotting analysis suggested that the EVs from uterine luminal fluid expressed three positive markers (CD9, CD81, and CD63) but not one negative marker (calnexin) (Fig. 3C includes cropped blot images, and the original blot image can be obtained from Figure S2). D Crossing points (y-axis) for hsa-miR-145-5p in cells, EVs extracted from primary hEEC cultures, supernatants, and total supernatants without depletion of EVs. The box indicates the first, median, and third quartiles, and the error bars indicate the minimum and maximum values. hEECs, human endometrial epithelial cells