Fig. 4

Effects of miR-145-5p mimic-enriched EVs on in vitro development of mouse IVF embryos. Mouse IVF embryos co-incubated with miR-145-5p mimic-enriched EVs or scramble miRNA EVs on day 1.5 of culture. The continuing culture was conducted for 72 h, and the development rate of the blastocyst was calculated. The proportions of day-1.5 embryos that developed to the blastocyst stage in the untreated control (n = 205), scramble-EVs (n = 162), and miR-145-5p mimic groups (n = 180) were 46.31% ± 4.23%, 45.25% ± 3.79%, and 14.13% ± 1.79%, respectively. All the experiments were repeated at least three times. Chi-square test, *P < 0.05; **P < 0.01; ***P < 0.001. EVs, extracellular vesicles