Fig. 5

Effects of miR-145-5p mimic-enriched EVs on the expression levels of Notch pathway components in mouse blastocyst-stage embryos. Mouse IVF embryos co-incubated with miR-145-5p mimic-enriched EVs or scramble miRNA EVs on day 1.5 of culture. The continuing culture was conducted for 72 h, and the mRNA expression profiles of 10 genes related to the Notch signalling pathway were measured by qRT-PCR. The histograms present four genes that encode proteins that are members or regulators of the Notch signalling pathway with markedly different expression levels (A) and six genes that encode proteins that are members or regulators of the Notch signalling pathway with nonsignificant differences in expression (B) between the miR-145-5p mimic (n = 173) and control (n = 119) groups. All the experiments were repeated at least three times. Unpaired t-test; *P < 0.05; **P < 0.01; ***P < 0.001. EVs, extracellular vesicles