Fig. 6

Effects of miR-145-5p mimic-enriched EVs on in vitro development of human IVF embryos. Human IVF embryos co-incubated with miR-145-5p mimic-enriched EVs or scramble miRNA EVs on day 2 of culture. The continuing culture was conducted for 96 h, and the development rate of the blastocyst was calculated. A Representative image of scramble miRNA EVs added to IVF embryos for 96 h. B Representative image of miR-145-5p mimic-enriched EVs added to IVF embryos for 96 h. C Proportions of embryos that developed into the blastocyst stage in the scramble-EVs (n = 45) and miR-145-5p mimic-EVs (n = 48) groups were 37.5% ± 3.95% and 18.75% ± 1.46%, respectively. All the experiments were repeated at least three times. Chi-square test, *P < 0.05; **P < 0.01; ***P < 0.001. EVs, extracellular vesicles