Fig. 7

Effects of miR-145-5p mimic-enriched EVs on the expression levels of NOTCH pathway components in human blastocyst-stage embryos. Human IVF embryos co-incubated with miR-145-5p mimic-enriched EVs or scramble miRNA EVs on day 2 of culture. The continuing culture was conducted for 96 h, and the mRNA expression profiles of 10 genes related to the NOTCH signalling pathway were measured by qRT-PCR. The histograms present three crucially differentially expressed genes that encode proteins that are members or regulators of the NOTCH signalling pathway (A) and seven genes that encode proteins that are members or regulators of the Notch signalling pathway with nonsignificant expression differences (B) between the miR-145-5p mimic (n = 48) and control (n = 45) groups. All the experiments were repeated at least three times. Unpaired t-test; *P < 0.05; **P < 0.01; ***P < 0.001. EVs, extracellular vesicles