Fig. 4

(A, C) Flow cytometry analysis of apoptosis using Annexin V-APC/PI staining. The percentage of apoptotic cells significantly increased in a dose-dependent manner, with the highest apoptosis rate observed in the 10 µM sempervirine treatment group; (B, D) Cell cycle distribution of SKOV3 cells treated with sempervirine. Flow cytometry results indicate a decrease in the G1 phase and an increase in the S and G2/M phases, suggesting cell cycle arrest at these stages. Data are presented as mean ± SD from three independent experiments