Fig. 6

Interference with CHD1L inhibited the proliferation, promoted the apoptosis and induced cycle arrest in OC cells by binding PLK1. The PLK1 overexpression plasmid was constructed and the transfection efficiency of Ov-PLK1 was detected by RT-qPCR (A) and Western blot (B). C and D. EDU staining detected the proliferation of CAOV-3 cells co-transfected with Ov-PLK1 and siRNA-CHD1L. E. TUNEL assay detected the apoptosis of CAOV-3 cells co-transfected with Ov-PLK1 and siRNA-CHD1L. F. Western blot detected the expression of apoptosis-related proteins Bcl-2, Bax, Cleaved caspase3 and caspase3 in CAOV-3 cells co-transfected with Ov-PLK1 and siRNA-CHD1L. G. Cycle distribution of CAOV-3 cells co-transfected with Ov-PLK1 and siRNA-CHD1L was determined by flow cytometry. H. Western blot detected the cycle-related proteins CDK1 and CyclinB1 in CAOV-3 cells co-transfected with Ov-PLK1 and siRNA-CHD1L. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001