Fig. 1
DHEA subcutaneous injection combined with HFD feeding induces the mice with PCOS combined with IR. For A-D, the mice were divided into two groups (Control, DHEA + HFD). For A-C, DHEA subcutaneous injection combined with HFD feeding significantly disrupted the estrous cycle of mice. (A) The estrous cycle of mice (n = 10 mice per group) was observed for 12 days. P: Proestrus, E: Estrus, M: Metestrus, D: Diestrus. (B) H&Estained smears of vaginal secretions at four stages of the estrous cycle in mice (magnification 100×). (C) Proportion of estrous cycle stages in mice. (D) Body Weight, FINS, HOMA-IR, Testosterone, E2, P, LH, LH/FSH (n = 8 mice per group). Data were analyzed by unpaired samples t-test and are expressed as mean ± SEM. *P < 0.05. **P < 0.001. ***P < 0.0001