Fig. 2

Wxzz treatment improved the estrous cycle, insulin and hormone level disorder of mice with PCOS and IR induced by DHEA and HFD. For A-C, the mice were divided into four groups (control, DHEA + HFD, DHEA + HFD + Met, DHEA + HFD + Wxzz). For A-C: Wxzz treatment significantly improved the estrous cycle disorder of the mice. (A) The estrous cycle of mice (n = 6 mice per group) was observed for 12 days. P: Proestrus, E: Estrus, M: Metestrus, D: Diestrus. (B) Proportion of estrous cycle stages in mice. (D) Body Weight, FINS, HOMA-IR, Testosterone, LH, LH/FSH (n = 6 mice per group). Data were analyzed using one-way ANOVA with Tukey’s multiple comparison post-hoc test and data are presented as means ± SEM. *P < 0.05. **P < 0.001. ***P < 0.0001