Fig. 4

Wxzz ameliorates pathological changes in gastrocnemius muscle structure in DHEA and HFD-induced PCOS combined with IR mice. Wxzz promotes protein and gene expression of AMPK, PGC1-α, FNDC5, and Irisin in gastrocnemius muscle of mice. (A) Representative H&E staining plots of gastrocnemius muscle (6 mice per group). Scale bar: 10 μm (magnification: 10 × 10). (B) Protein expression levels of AMPK, PGC1-α, FNDC5, Irisin in gastrocnemius muscle tissues. (C) Protein quantification with AMPK, PGC1-α, FNDC5, Irisin. Relative mRNA levels of AMPK, PGC1-α, FNDC5, Irisin genes in gastrocnemius muscle tissues. Data were analyzed by one-way ANOVA and Tukey’s multiple comparisons post hoc test and are expressed as mean ± SEM. *P < 0.05. **P < 0.001. ***P < 0.0001