Fig. 6

Wxzz ameliorates pathological changes in abdominal adipose structure in DHEA and HFD-induced PCOS combined with IR mice. Wxzz promotes protein and gene expression of CaMKK, AMPK, PGC1-α, and UCP1 in abdominal adipose of mice. (A) Representative oil red O staining plots of adipose tissue (6 mice per group). Scale bar: 10 μm (magnification: 10 × 10). (B) Protein expression levels of CaMKK, AMPK, PGC1-α, UCP1 in adipose tissue. (C) Protein quantification with CaMKK, AMPK, PGC1-α, UCP1. Relative mRNA levels of CaMKK, AMPK, PGC1-α, UCP1 genes in adipose tissue. Data were analyzed by one-way ANOVA and Tukey’s multiple comparisons post hoc test and are expressed as mean ± SEM. *P < 0.05. **P < 0.001. ***P < 0.0001