Woxuanzhongzhou formula improves DHEAS and high-fat diet-induced IR and anovulatory mice via AMPK/PGC1- α/Irisin pathway

Table 3 Primer sequence used for RT-PCR

Gene	Primer	Sequence	Size(bp)	GeneBank ACC
AMPK	Forward	5’ AAACCCACAGAAATCCAAAC 3’	114	NM_001013367.3
AMPK	Reverse	5’ TGCTTGATTGCTCTACAAAC 3’	114	NM_001013367.3
PGC1-α	Forward	5’ TGGATTGAAGTGGTGTAG 3’	180	NM_001402987.1
PGC1-α	Reverse	5’ GTCAGTGCATCAAATGAG 3’	180	NM_001402987.1
FNDC5	Forward	5’ CATTGTTGTGGTCCTCTTC 3’	222	NM_027402.4
FNDC5	Reverse	5’ CGCATTCTCTACTGTCTTC 3’	222	NM_027402.4
Irisin	Forward	5’ GAGCCCAATAACAACAAG 3’	236	NM_006503212.5
Irisin	Reverse	5’ AATAAGCCCGATGATAGG 3’	236	NM_006503212.5
CaMKK	Forward	5’ GTCCACAGGGACATCAAG 3’	203	NM_001362841.1
CaMKK	Reverse	5’ GTGGCCCATACATCCAAG 3’	203
UCP1	Forward	5’ CATTCAGAGGCAAATCAG 3’	135	NM_009463.3
UCP1	Reverse	5’ ACACCTCCAGTCATTAAG 3’	135	NM_009463.3
GAPDH	Forward	5’ ATCACTGCCACCCAGAAG 3’	191	NM_008084.2
GAPDH	Reverse	5’ TCCACGACGGACACATTG 3’	191	NM_008084.2

ISSN: 1757-2215