Fig. 3

NEAT1 regulates KGN cell proliferation through miR-29a-3p. A qRT-PCR analysis of miR-29a-3p expression levels in KGN cells transfected with miR-29a-3p mimic, NEAT1 + mimic NC, and NEAT1 + miR-29a-3p mimic; B qRT-PCR analysis of CCNB1 and CDK2 mRNA levels in KGN cells transfected with different constructs; C Western blot analysis showing the effects of miR-29a-3p and NEAT1 on CCNB1 and CDK2 protein expression; D Quantification of CCNB1 and CDK2 protein levels from Western blot analysis; E CCK-8 results showing the effects of miR-29a-3p and NEAT1 on KGN cell proliferation; F Flow cytometry plots showing cell cycle distribution in KGN cells under different treatment conditions, with quantification of cell cycle phase distribution (G0/G1, S, G2/M) shown in the bar graph. Data is presented as mean ± SD. *p < 0.05 and **p < 0.01 compared to control groups