Fig. 5

miR-29a-3p regulates KGN cell proliferation through IGF1. A qRT-PCR analysis of IGF1, CCNB1, and CDK2 mRNA expression levels in KGN cells transfected with miR-29a-3p mimic, IGF1 + mimic NC, or IGF1 + miR-29a-3p mimic; B Western blot analysis of IGF1, CCNB1 and CDK2 protein levels in KGN cells transfected with miR-29a-3p mimic, IGF1 + mimic NC, or IGF1 + miR-29a-3p mimic; C Quantification of IGF1, CCNB1, and CDK2 protein levels from Western blot analysis; D CCK-8 results showing the effects of miR-29a-3p and IGF1 on KGN cell proliferation; E Flow cytometry plots showing cell cycle distribution in KGN cells under different treatment conditions (miR-29a-3p mimic with or without IGF1 overexpression), with quantification of cell cycle phase distribution (G0/G1, S, G2/M) shown in the bar graph. Data is presented as mean ± SD. *p < 0.05 and **p < 0.01 compared to control groups