Table 1 Tissue viability values (%) before freezing and at thawing (0 h. 2 days. and 7 days of culture). Values expressed as mean ± standard error. Different letters within the same column indicate significant differences between treatments (p < 0.05)

TrypLE

76.89 ± 0.21^a

77.85 ± 0.9^a

67.24 ± 0.08^a

67.26 ± 0.42^a

Collagenase

81.76 ± 0.5^b

66.71 ± 0.25^b

66.76 ± 0.2^b

53.32 ± 0.23^c

Dispase

87.58 ± 0.05^c

65.27 ± 0.2^c

62.04 ± 0.3^c

40.57 ± 0.26^d

Accutase

71.08 ± 0.44^d

47.79 ± 0.54^d

56.03 ± 0.35^d

45.06 ± 0.16^d

Hyaluronidase

71.49 ± 0.2^d

70.25 ± 0.3^e

61.81 ± 0.46^b

59.69 ± 0.21^b

Control

74.01 ± 034^f

70.32 ± 0.33^e

61.52 ± 0.22^b

60.06 ± 0.25^b

P-value

 < 0.001

 < 0.001

 < 0.001

 < 0.001