Fig. 5
From: Nicotinamide riboside supplementation ameliorates ovarian dysfunction in a PCOS mouse model
NR supplementation decreased ROS levels and increased mitochondrial membrane potential in ovarian stromal cells of PCOS mice. A Ovarian stromal cells from three groups of mice were stained with MitoSOX to assess ROS levels using FCM. B The percentages of ovarian stromal cells with high ROS levels (n = 9–15 replicates of cells pooled from 3 mice). C Ovarian stromal cells from three groups of mice were stained with JC-1 to assess mitochondrial membrane potential using FCM. The x-axis represents JC-1 green fluorescence, while the y-axis represents JC-1 red fluorescence. D The ratios of red to green fluorescence in ovarian stromal cells (n = 11 replicates of cells pooled from 3 mice). Data are presented as the mean ± SD. **P < 0.01 and ***P < 0.001 by one-way ANOVA compared to DHEA-treated mice