Fig. 1

Effect of UGT2B15 on the DHT accumulation in KGN cells. A Excessive DHT (500 nM) exposure on gene expression of the UGT2Bs family in KGN cell. B Excessive DHT (500 nM) exposure on protein expression of UGT2B15 in KGN cells. C Effect of siRNA interferences on UGT2B15 at gene level. D Effect of siRNA interferences on UGT2B15 at the protein level. E Verify the regulative effects of (R)-NAF and (S)-NAF on UGT2B15 mRNA expression levels. F Verify the regulative effects of (R)-NAF and (S)-NAF on UGT2B15 protein expression levels. G UGT2B15 KD and (R)/(S)-NAF played opposite roles in the regulation of DHT accumulation induced by excessive T (500 nM) exposure. Results of all qPCR and Western blot were standardized based on GAPDH levels and are expressed in comparison to the vehicle group. Values are expressed as mean ± SD (n = 3–5 per group). P values were determined by one-way ANOVA with Tukey's multiple comparison post-hoc test. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ns means no significant difference