Fig. 3

UGT2B15 ameliorated the apoptosis induced by androgen excess in KGN cells. A UGT2B15 KD and NAF enantiomers had contrasting effects on reducing the apoptosis of KGN cells caused by excessive DHT. B UGT2B15 KD and NAF enantiomers had contrasting effects on the modulation of Caspase10, Cleaved caspase3, Bcl-2/Bax gene expression induced by excessive DHT. All qPCR and Western blot results were standardized based on GAPDH levels and are presented in comparison to the control group. Values are expressed as mean ± SD (n = 3 per group). P values were determined by one-way ANOVA with Tukey's multiple comparison post-hoc test. P values less than 0.05, 0.01, and 0.001 are denoted as *, **, and ***, respectively, while ns indicates no significant distinction