Fig. 5

NR1H4 regulates UGT2B15 transcription and its interaction is influenced by DHT and NAF treatments. A JASPAR online analysis predicted NR1H4 binding sites in the promoter region of the human UGT2B15 gene (http://jaspar.genereg.net). B Dual-luciferase reporter assays were performed to validate the connection between UGT2B15 gene promoter regions and NR1H4, and the effects of DHT, (R)-NAF, and (S)-NAF on this process. C Chromatin immunoprecipitation (ChIP) study. D Effects of DHT on AR, NR1H4, and UGT2B15 protein levels in KGN cells. E Effects of (R)-NAF and (S)-NAF on NR1H4 protein levels in KGN cells. All qPCR and Western blot results were standardized based on GAPDH levels and are presented in comparison to the control group. Values are expressed as mean ± SD (n = 3–5 per group). P values were determined by one-way ANOVA with Tukey's multiple comparison post-hoc test. *P and ^#P < 0.05, **P and ^##P < 0.01, ***P and ^###P < 0.001, ns means no significant difference