Table 1 Sequences of the primers used for RT-qPCR
Gene | Human | Rat |
|---|---|---|
IL-1β | F: 5’-CAGAAGTACCTGAGCTCGCC-3’ | F: 5’-GACTTCACCATGGAACCCGT-3’ |
R: 5’-TGAAGCCCTTGCTGTAGTGG-3’ | R: 5’-GGAGACTGCCCATTCTCGAC-3’ | |
IL−6 | F: 5’-ACCGGGAACGAAAGAGAAGC-3’ | F: 5’-ACAGCGATGATGCACTGTCA-3’ |
R: 5’-TCTCCTGGGGGTATTGTGGA-3’ | R: 5’-AGCACACTAGGTTTGCCGAG-3’ | |
iNOS | F: 5’-GGCAGCCTGTGAGACCTTG-3’ | F: 5’-ATCTTTGGCCCGAAGGTCG-3’ |
R: 5’-GCATTGGAAGGAAGGAGCGTTTC-3’ | R: 5’-AGTCACATGCAGCTTGTCCA-3’ | |
PTGS2 | F: 5’-GTTCCACCCGCAGTACAGAA-3’ | F: 5’-ATTACTGCTGAAGCCCACCC-3’ |
R: 5’-CTCCACCAAAAGTGCTTGGC-3’ | R: 5’-GGCCCTGGTGTAGTAGGAGA-3’ | |
MMP9 | F: 5’-TTCAGGGAGACGCCCATTTC-3’ | F: 5’-GATCCCCAGAGCGTTACTCG-3’ |
R: 5’-TCGCTGGTACAGGTCGAGTA-3’ | R: 5’-GTTGTGGAAACTCACACGCC-3’ | |
MCL1 | F: 5’-GCCTTCCAAGGATGGGTTTG-3’ | F: 5’-AGATCATCTCCCGCTACCTG-3’ |
R: 5’-AGGTTGCTAGGGTGCAACTC-3’ | R: 5’-GCCCCAGTTTGTTACGCCAT-3’ | |
JUN | F: 5’-GCCAGGTCGGCAGTATAGTC-3’ | F: 5’-CCAACCAACGTGAGTGCAAG-3’ |
R: 5’-GGACTCTGCCACTTGTCTCC-3’ | R: 5’-GAGGGCATCGTCGTAGAAGG-3’ | |
GAPDH | F: 5’-TCCGTGGTCCACGAGAACT-3’ | F: 5’-GGACCAGGTTGTCTCCTGTG-3’ |
R: 5’-GAAGCATTTGCGGTGGACGAT-3’ | R: 5’-ATTCGAGAGAAGGGAGGGCT-3’ |