Fig. 3

TRPS1 expression in ovarian clear cell carcinoma cells. A-B: Western blot (WB) and real-time quantitative PCR (RT-qPCR) were used to measure TRPS1 expression in TOV21G and ES-2 cells. C-D: TRPS1 overexpression efficiency in ES-2 cells was confirmed using RT-qPCR and Western blot after transfection with the TRPS1 overexpression plasmid. E-F: Knockdown efficiency in TOV21G cells transfected with TRPS1-siRNA1 or TRPS1-siRNA2 was validated by RT-qPCR and Western blot. (*p < 0.05, **p < 0.01, ***p < 0.001)