Fig. 2

Cytokines in the pre-ovulatory microenvironment and serum (A) The expression of IL4, IL5, IL13, IL17, IL6, IL10, TNFA and IFNG mRNA isolated from lymphocytes collected from follicular aspirates within the preovulatory microenvironment was assessed using RT-qPCR. Each data point represents an individual subject, data are expressed as mean ± SEM. * p < 0.05, ns, not significant. mRNA level from OW group compared to NW group (Mann-Whitney U Test). (B) The heatmap of cytokines detection in follicular fluids and serum. The levels of the cytokines were measured using Luminex 200 platform