Fig. 1

Characterization of exosomes from ovarian cancer cells at different culture time points. (A) A schematic diagram illustrating the experimental design from sample collection till sequencing. (B) TEM image of the exosomes. Electron microscopy allowed visualizing membrane-bound nano vesicles sized ~ 100 nm. Scale bar = 100 nm. (C) Analysis of exosomes with NanoSight LM10-HS instrument. (D) Exosome validation by nanoflow fluorescence indicating the CD9, CD63 and CD81 protein markers for exosomes