Fig. 2

Identification of exosomal miRNAs by miRNA sequencing. (A) Hierarchical clustering heatmap analysis for expression pattern of miRNAs from the four (T12, T24, T36 and T48) groups. (B) Venn diagrams of the identified miRNAs from the four (T12, T24, T36 and T48) groups. (C) The miRNA expression were determined by high-throughput sequencing. Transcripts per million (TPM) per million mapped reads was used to calculate the expression levels of miRNAs. (D) The miRNA expression levels of target genes were determined by RT-qPCR and normalized against U6 expression level. Statistical analysis was performed by Student’s t-test and data are presented at the mean ± standard deviation of three replicates. *P < 0.05, **P < 0.01. RT-qPCR, reverse transcription quantitative real-time polymerase chain reaction