Fig. 3

TAM-derived exosomal miR-589-3p enhances OC cell proliferation and depresses apoptosis. Exosomes were extracted after transfection of miR-589-3p inhibitor or NC inhibitor in TAM and then SKOV3 and CAOV3 cells were treated with exosomes. (A) qRT-PCR was performed to detect the expression of miR-589-3p after co-culture of TAM-derived exosomes with SKOV3 and CAOV3 cells; (B) qRT-PCR was performed to detect the level of exosomal miR-589-3p after transfection with miR-589-3p inhibitor in M2 macrophages; (C) qRT-PCR was applied to detect the level of miR-589-3p in cells after treatment of SKOV3 and CAOV3 cells with exosomes transfected with miR-589-3p inhibitor; (D) CCK-8 experiment was performed to measure the changes in SKOV3 and CAOV3 cell viability at 0 h, 24 h, 48 h and 72 h after treatment of SKOV3 and CAOV3 cells with exosomes transfected with miR-589-3p inhibitor; (E) Flow cytometry was performed to detect the changes in apoptotic capacity after treatment of SKOV3 and CAOV3 cells with exosomes transfected with miR-589-3p inhibitor; (F) The quantification of apoptosis rate in SKOV3 and CAOV3 cells