Fig. 4

BCL2L13 is a downstream target of miR-589-3p. (A) Targetscan and miRDB were used to predict the miR-589-3p target genes, and apoptosis-related genes were selected to take the intersection; (B) qRT-PCR was performed to detect the effect of miR-589-3p knockdown on the expression of the above genes; (C) Targetscan website was applied to predict the binding site between miR-589-3p and BCL2L13; (D) Luciferase reporter assay was used to verify the binding relationship between miR-589-3p and BCL2L13; (E) Ago2-RIP- PCR was used to verify the binding relationship between miR-589-3p and BCL2L13; (F) qRT-PCR and western blot was used to detect the level of BCL2L13 in TAM-derived exosomes co-cultured with SKOV3 and CAOV3 cells