Fig. 5

BCL2L13 silencing reverses the effect of knockdown of miR-589-3p on cell progression. (A) qRT-PCR and western blot was applied to detect the changes of BCL2L13 expression after transfection with si-NC, si-BCL2L13#1, si-BCL2L13#2 in SKOV3 and CAOV3 cell lines; (B) CCK-8 was used to detect the changes of SKOV3 and CAOV3 cell viability at 0 h, 24 h, 48 h, and 72 h after knockdown of miR-589-3p and/or knockdown of BCL2L13 treatment in SKOV3 and CAOV3 cells; (C) Flow cytometry was performed to detect the effect of knockdown of miR-589-3p and /or knockdown BCL2L13 treatment on SKOV3 and CAOV3 cell apoptosis; (D) The quantification of apoptosis rate in SKOV3 and CAOV3 cells