Fig. 2

Comparison of T cell subsets in PCOS endometrium and normal endometrium by flow cytometry. (A) Pseudocolor plots showing the gating strategy for CD4⁺CD8⁻ T cells, CD4⁻CD8⁺ T cells, Th1, Th2, Th17, and Treg cells. Single cells were gated to eliminate debris and clumped cells. The exclusion of CD45-negative cells was followed by the identification of lymphocytes. After CD3⁺T cells were identified, CD4⁺CD8⁻ T cells and CD4⁻CD8⁺ T cells were examined. CD4⁺ CD8⁻ T cells were selected, and Treg, Th1, Th2, and Th17 cells were identified successively. Values inside the plots represent the percentages from the parent gate. SSC-A: side scatter area, FSC-A: forward scatter area, FSC-H: forward scatter height. (B) Comparison of CD4⁺CD8⁻ T cells and CD4⁻CD8⁺ T cells in PCOS endometrium and normal endometrium. (C) Comparison of Th1 and Th2 cells in PCOS endometrium and normal endometrium. (D) Comparison of Th17 and Treg cells in PCOS endometrium and normal endometrium. (E) Comparison of IFNG and TNF in PCOS endometrium and normal endometrium (F) Comparison of CD4 + CD8- T cells and CD4-CD8 + T cells in the endometrium of controls and the four phenotypic subgroups of PCOS patients. (G) Comparison of Th1 and Th2 cells in the endometrium of controls and the four phenotypic subgroups of PCOS patients. (H) Comparison of Th17 and Treg cells in the endometrium of controls and the four phenotypic subgroups of PCOS patients. (*) P < 0.05 by Mann-Whitney U test