Fig. 5
From: Downregulation of FASN in granulosa cells and its impact on ovulatory dysfunction in PCOS
A-C Investigation of the impact of FSK (10 uM, 4 h) on the expression of FASN, C/EBPα, and C/EBPβ mRNA in KGN cells. D Effects of FSK(10 u M,4 h) on protein levels of FASN, C/EBPα, C/EBPβ in KGN cells. E With or without FASN knockdown, the impact of FSK (10 uM, 4 h) on the phosphorylation of ERK1/2 in KGN cells. F Effects of FSK (10 uM, 4 h) on ERK1/2 phosphorylation, C/EBPα, C/EBPβ in the presence and absence of inhibitor for ERK1/2 (U0126, 10 uM). G KGN cells treated with FSK(10 uM,4 h) were fixed and labelled with anti-FASN antibodies (red). The cellular cores were labeled with 40,6-diamidino-2-phenylindole (DAPI) dye, resulting in a blue coloration. The photomicrographs accurately depict the results of three separate investigations. The analysis of relative fluorescence intensity was conducted using the ImageJ program. The figures display representative blots, data were analyzed using unpaired samples t-test or one-way ANOVA with Tukey’s multiple comparison post-hoc test and the results presented are the means ± standard error of the mean (SEM) obtained from 3 to 5 tests. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 compared to the control group