Fig. 6

Lentivirus vectors promote downregulation of FASN, leading to ovulatory failure in rats. A Ovarian weight of rats before superovulation (n = 6 per group). B Ovarian sections were frozen before superovulation. Cell nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) (blue). Lentivirus showed green fluorescence that could be seen by means of a fluorescent scope.The photomicrographs were typical of those found in three separate studies. C hCG16h, protein levels of FASN, C/EBPα, C/EBPβand ERK1/2 phosphorylation was examined in ovaries from two groups(n = 3 per group). D Oocytes numbers per ovary (n = 6 for every group). E Rats injected with the control vector (top) and the FASN-lentivirus vector (bottom) showed different ovarian morphologies had distinct ovarian morphologies after superovulation (PMSG48h + hCG16 h).The asterisk indicates the corpus luteum. Scale bar: 500 m. Data were analyzed using unpaired samples t-test and are presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 vs. the control group