Fig. 3

Heterogeneity of tumor cells in SCCOHT and expression patterns in tumor progression. A, The UMAP plot demonstrates the subclusters of cancer cells in primary and recurrent tumor specimens, and the proportion of cell components in specific patient samples is displayed next to it. B, Expression levels of representative well-known markers across the different clusters in cancer cells. Color key from gray to red indicates relative expression levels from low to high. C, Heatmap showing the enriched hallmark gene signatures of heterogeneity of cells within tumors. D, The evolutionary trajectories of these cancer cells. E, Box plot comparing the stemness scores in the different clusters. F, Expression changes of characteristic genes along the pseudotime. G, Heatmap showing the dynamic changes in gene expression along the pseudotime and differential genes and pathways are labeled