Fig. 5

The characteristics of T cells in SCCOHT. A, The UMAP diagram shows different components of T cells identified in three samples. B, Dot plots indicating the differentially expressed genes in eight T cell subtypes. C, The portion of T cell subtypes in the three samples. D, the heatmap displays the gene expression characteristics of various T cell subpopulations analyzed by GSVA. E, Representative images of mIF staining with the PD-1, CD4, and CD8 antibodies of normal ovarian tissue, primary lesion, and recurrent lesion (Scale bars, 200 μm). Enlarged outset shows the infiltration of T cells expressing various marker genes and cells co-expressing PD-1and CD4/CD8 (Scale bars, 100 μm). F, CD4-positive, CD8-positive, PD-1-expressing CD4-positive and PD-1-expressing CD8-positive cell counts per mm². The y-axis indicates number of marker-positive cells, and the x-axis indicates SCCOHT cases. Percentage of PD-1-positive cells out of all CD4/CD8 cells. The y-axis indicates percentage of PD-L1-expressing T cells, and the x-axis indicates SCCOHT cases (*P<0.05). G, the heatmap displays the interaction between two cell types, with darker colors indicating a greater number of interactions between the two cell types. H, the bubble plot illustrated the predicted chemokine interactions between macrophages as ligand cells and other cell types