Fig. 1
From: Icariside II induces ferroptosis through the down-regulation of SLC7A11 in ovarian cancer
Effects of Icariside II on SKOV3 cells ferroptosis. SKOV3 cells were exposed to various concentrations of Icariside II (0, 20, 40, 60 µM) for 48 h. (A and B) Statistical analysis of intracellular ROS content. Determination of ferrous (C) and total iron (D) levels in SKOV3 cells. (E and F) Levels of Ptgs2 and Chac1 were detected using qRT-PCR. Analysis of the levels of Cys (G), GSH (H), and GPX4 (I) in SKOV3 cells. (J and K) SLC7A11 levels were determined using RT-qPCR and Western blot analysis. * P < 0.05, **P < 0.01 vs. Control. ROS, reactive oxygen species; Cys, cysteine; GSH, glutathione; GPX4, glutathione peroxidase 4