Fig. 2
From: Icariside II induces ferroptosis through the down-regulation of SLC7A11 in ovarian cancer
Effects of SLC7A11-plasmid on Icariside II-induced ferroptosis in SKOV3 cells. (A-B) SLC7A11 levels in the control, control-plasmid, and SLC7A11-plasmid groups were assessed using RT-qPCR and Western blotting. (C-D) SLC7A11 levels in the control, Icariside II, Icariside II + control-plasmid, and Icariside II + SLC7A11-plasmid groups were analyzed using RT-qPCR and Western blotting. (E) Lipid ROS production in SKOV3 cells was evaluated using flow cytometry. Intracellular Fe2+ (F) and total iron (G) levels in SKOV3 cells were determined. (H, I) qRT-PCR analysis of Ptgs2 and Chac1 levels. Cys (J), GSH (K), and GPX4 (L) levels in the SKOV3 cells. **P < 0.01 vs. control-plasmid; ## P < 0.01 vs. Control; &,&& P < 0.05, 0.01 vs. Icariside II + control-plasmid. ROS, reactive oxygen species; Cys, cysteine; GSH, glutathione; GPX4, glutathione peroxidase 4