Fig. 1
Conditional knock-in of Foxj2 in mouse ovary granulosa cells. A Breeding strategy for Amh-cre; Foxj2tg/tg (Cre tgtg) mice. B, C Genotypes of the mice: 1: Amh-cre; Foxj2tg/tg; 2: Foxj2tg/tg; 3, 5: Amh-cre; Foxj2tg/+; 4: Foxj2tg/+ mice. D Relative mRNA expression levels of Foxj2 in different mouse tissues between the Amh-cre; Foxj2tg/tg and the control mice quantified by qRT-PCR. Data are presented as the mean ± SEM (n = 3), normalized to Actb (β-Actin) transcript levels. *P < 0.05. E, F Representative immunohistochemical staining images of FOXJ2 in the ovaries of Amh-cre; Foxj2tg/tg and control mice (E), along with an analysis of the average optical density (F). Scale bar: 50 μm. Data are presented as the mean ± SEM (n = 4). **P < 0.01. G qRT-PCR analysis of Foxj2 mRNA levels in the isolated ovarian granulosa cells between Amh-cre; Foxj2tg/tg and control mice. Data are presented as the mean ± SEM (n = 12), normalized to Actb (β-Actin) transcript levels. **P < 0.01. H, I Representative western blot images (H) with analysis of grey values (I) of FOXJ2 protein levels in the isolated ovarian granulosa cells between Amh-cre; Foxj2tg/tg and control mice. β-Actin was used as a loading control. Data are presented as the mean ± SEM (n = 4). **P < 0.01