Fig. 2
The premature ovarian insufficiency (POI)-like phenotype of Amh-cre; Foxj2tg/tg female mice. A Gross morphology of the ovaries from control and Amh-cre; Foxj2tg/tg females (Cre-tgtg). B Ratio of the ovary to body weight from control and Cre-tgtg females. Data are presented as the mean ± SEM (n = 3 or 4). *P < 0.05. C Pups per litter sired by control and Cre-tgtg females. Data are presented as the mean ± SEM (n = 6 or 10). ****P < 0.0001. D Interval days between the first and second litter from control and Cre-tgtg females. Data are presented as the mean ± SEM (n = 3 or 4). **P < 0.01. E Representative images of vaginal smears showing different stages of the estrous cycle of mice. Scale bar: 50 μm. F Typical changes observed in an estrous cycle during a 17-day period in control and Cre-tgtg females. Abbreviations: P - Proestrus, E - Estrus, M - Postestrus, D - Diestrus. G Proportion of each stage of the estrous cycle over a 17-day period in control and Cre-tgtg females. Data are presented as the mean ± SEM (n = 5). ****P < 0.0001. H The average length of the estrous cycle. Data are presented as the mean ± SEM (n = 5). ****P < 0.0001. I Representative images of MII oocytes collected from one ovary of 3-week-old control and Amh-cre; Foxj2tg/tg females. Scale bar: 100 μm. J The number of MII oocytes from each pair of ovaries of 3-week-old control and Amh-cre; Foxj2tg/tg females. Data are presented as the mean ± SEM (n = 7 or 3). ***P < 0.001. K, L and M Levels of follicle stimulating hormone (FSH), estrogen(E2), and anti-mullerian hormone (AMH) in serum, measured by ELISA, in control and Cre-tgtg females. Data are presented as the mean ± SEM (n = 15). ***P < 0.001. ****P < 0.0001