Fig. 5
MCU is upregulated in GCs of Amh-cre; Foxj2tg/tg mice and is a target of FOXJ2. A A Venn diagram illustrating differentially expressed genes (DEGs) (purple) and the predicted target genes of FOXJ2 (yellow) using the ARACNe algorithm. B Validation of DEGs related to the mitochondrial calcium uniporter complex using qRT-PCR assay. Data are presented as the mean ± SEM (n = 4). *P < 0.05; ***P < 0.001. C Expression and co-localization of MCU and FOXJ2 in GCs using immunofluorescent staining (IF). Scale bar: 50 μm. D, E The binding sites of FOXJ2 to Mcu promoter region in GCs from Amh-cre; Foxj2tg/tg mice analyzed by ChIP-PCR using an anti-HA-tag antibody. Data are presented as the mean ± SEM (n = 3). **P < 0.01. F Verification of FOXJ2 binding to the Mcu promoter using a Dual-luciferase reporter assay. Data are presented as the mean ± SEM (n = 3). *P < 0.05, **P < 0.01