Fig. 6
Targeting MCU rescues FOXJ2 overexpressioninduced mitochondrial calcium overload and apoptosis in GCs. A A representative image of Rhod-2AM loaded GCs in each group. Scale bar: 50 μm. B Quantitative analysis of the mean fluorescence intensity of Rhod-2AM (indicating mitochondrial Ca2+ levels) in GCs from each group. Data are presented as the mean ± SEM (n = 44–55). ****P < 0.0001. C Representative images of Rhod-2AM loaded GCs in each group at the initial recording time, 5 s, and 2 min after exogenous Ca2+ loading. Scale bar: 5 μm. D Time-course records of Rhod-2AM fluorescence intensity in GCs from each group. Data are presented as the mean ± SEM (n = 4). E, F Representative flow cytometry images (E) and quantitative analysis (F) of apoptosis levels in GCs from each group. Data are presented as the mean ± SEM (n = 4). * P < 0.05. **P < 0.01. ***P < 0.001. ****P < 0.0001