Fig. 1

THBS1 is significantly overexpressed in OC and correlates with poor prognosis. (A) Comparison of THBS1 expression levels between OC tissues (TCGA-OV dataset) and normal ovarian tissues (GTEx dataset). (B) THBS1 expression levels in normal ovarian epithelial cells versus OC cell lines (GSE14407 dataset). (C) Association between THBS1 expression levels and poor prognosis in OC patients (TCGA-OV dataset). (D) IHC analysis of THBS1 staining intensity in tumor and adjacent normal tissues from 97 OC patients. (E–G) Correlation of THBS1 staining intensity with clinical stage, tumor differentiation, and lymph node metastasis in 97 OC patients. (H) qPCR analysis of THBS1 mRNA expression levels in the ovarian epithelial cell line IOSE and OC cell lines SKOV3, A2780, and OVCAR3. Data are presented as violin plots, with each point representing an experimental replicate or patient sample. Statistical significance was determined using Student’s t-test or one-way ANOVA, followed by Tukey’s multiple comparison test (P < 0.05)