Fig. 4

THBS1 suppresses inflammation and immunosurveillance in an OC MTO mouse model. (A) Workflow illustrating the transplantation of ID8 OC cells into wild-type (WT) and THBS1 knockout (KO) mice. (B) IHC analysis of Ki67 and cleaved caspase-3 (C-Cas-3) staining intensities in tumor tissues. (C) Immunofluorescence analysis of VEGFA expression in tumor tissues. (D) IHC analysis of CD206 staining intensity in tumor tissues. (E–F) IHC analysis of CD4 + and CD8 + T cell infiltration in tumor tissues. (G–H) IHC analysis of PD-L1 and GAL-3 staining intensities in tumor tissues. Data are presented as violin plots, with each point representing a single mouse. Statistical significance was determined using two-way ANOVA, followed by Tukey’s multiple comparison test (P < 0.05)