Fig. 6

THBS1 expression is transcriptionally regulated by SNF2H. (A) Prediction of an SNF2H-binding motif near the THBS1 transcription start site using the JASPAR database. (B) Comparison of SNF2H expression levels between OC tissues (TCGA-OV dataset) and normal ovarian tissues (GTEx dataset). (C) Association of SNF2H expression with poor prognosis in OC patients (TCGA-OV dataset). (D) IHC analysis of SNF2H staining intensity in tumor and adjacent normal tissues from 97 OC patients. (E) Spearman correlation analysis of THBS1 and SNF2H staining intensities. (F) Pearson correlation analysis of THBS1 and SNF2H expression levels in the TCGA-OV dataset. (G) qPCR analysis of SNF2H and THBS1 mRNA expression after SNF2H knockdown in SKOV3 and A2780 cells. (H) ChIP analysis with anti-SNF2H showing enrichment of THBS1 promoter fragments. Data are presented as violin plots, with each point representing an experimental replicate or patient sample. Statistical significance was determined using Student’s t-test or one-way ANOVA, followed by Tukey’s multiple comparison test (P < 0.05)