Fig. 3

Tanshinone IIA restores TOCP-induced sex hormone disorders and related protein expression in female mice. Female mice were allocated into four groups: Control, TOCP 200 mg/kg/d, Tanshinone IIA 25 mg/kg/d + TOCP 200 mg/kg/d, and Tanshinone IIA 50 mg/kg/d + TOCP 200 mg/kg/d.(A–C) Serum levels of follicle-stimulating hormone (FSH), anti-Müllerian hormone (AMH), and estradiol (E2) were quantified via ELISA (n = 3). (D) Immunohistochemical analysis of AMH and FSHR (follicle-stimulating hormone receptor) expression in ovarian tissues (n = 3). (E–F) Western blot detection of AMH and FSHR protein levels, with β-actin as the loading control. F presents the quantitative analysis of AMH and FSHR, expressed as fold change relative to the control group (n = 3). The data are presented as mean ± SEM from at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001