Fig. 4

Tanshinone IIA Ameliorates TOCP-Induced Impairment of Granulosa Cell Viability, Proliferation, and Steroidogenic Enzymes. A. CCK- 8 assay showing cell viability in control, 0.125 mM, 0.25 mM, and 0.5 mM TOCP-treated groups at 24 h, 48 h, and 72 h. B. CCK- 8 analysis of cell viability in control, 0.5 mM TOCP, and 0.5 mM TOCP + tanshinone (0.001 mM, 0.01 mM) groups at 72 h. C-D. EDU staining quantification (C) and representative images (D) showing cell proliferation in control, 0.5 mM TOCP, and 0.5 mM TOCP + tanshinone IIA (0.001 mM, 0.01 mM) groups. E. Western blot analysis of CYP19 A1 and STAR protein expression normalized to β-actin in control, TOCP (0.5 mM), and tanshinone IIA (0.01 mM,0.001 mM) groups. F. Quantification of STAR protein levels in control, TOCP (0.5 mM), and tanshinone IIA (0.01 mM,0.001 mM) groups. The data are presented as mean ± SEM from at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001