Fig. 6

Tanshinone IIA inhibits TOCP-induced apoptosis. (A) The apoptosis level of ovaries treated with a vehicle control, 200 mg/kg/d TOCP, 200 mg/kg/d TOCP + 25 mg/kg/d tanshinone IIA or 200 mg/kg/d TOCP + 50 mg/kg/d tanshinone IIA was determined using TUNEL staining(n = 3). Scale bars represent 100 μm. (B) The protein expression levels of BAX and BCL- 2 were examined using western blot analysis(n = 3). (C) Quantification of the BAX/BCL- 2 ratio using western blot analysis(n = 3). The data are presented as mean ± SEM from at least three independent experiments. *p < 0.05, **p < 0.01