Fig. 8

Tanshinone IIA induces the activation of the AKT, P38, and ERK1/2 signaling pathways. Western blot analysis for AKT and P-AKT expression levels in ovaries were treated with a vehicle control, 200 mg/kg/d TOCP, 200 mg/kg/d TOCP + 25 mg/kg/d tanshinone IIA or 200 mg/kg/d TOCP + 50 mg/kg/d tanshinone IIA. (A) Western blot analysis for P-AKT and AKT expression levels. (B) Quantification of P-AKT/AKT expression level using western blot analysis (n = 3).(C)Western blot analysis for P38 and P-P38 expression levels. (D) Quantification of P-P38/P38 expression level using western blot analysis (n = 3). (E) Western blot analysis for ERK1/2 and P-ERK1/2 expression levels (n = 3). (F) Quantification of P-ERK1/2/ERK1/2 expression level using western blot analysis (n = 3). (G) Molecular docking site and three-dimensional force analysis diagram of tanshinone IIA and ERK, P38, AKT (n = 3). The data are presented as mean ± SEM from at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001