Skip to main content
Fig. 3 | Journal of Ovarian Research

Fig. 3

From: A compound heterozygous mutation in ZP1 and two novel heterozygous cis mutations in ZP3 causes infertility in women presenting with empty follicle syndrome

Fig. 3

Characterization of mutant ZP1 and ZP3 proteins. (A-C) Effects of mutants on ZP1 and ZP3 proteins levels in Hela cells transfected with wild-type or mutant plasmids were detected by WB. The bar graph (below) represents an optical densitometric plot of relative protein expression, with GAPDH used as a standard. Data are represented as the means ± SEMs, n = 3. **P < 0.01, ****P < 0.0001. (D) Effects of ZP1 mutations (p.Gly477* and p.Met1?) on intracellular localization and expression of ZP1 proteins by IF staining. Confocal microscopy image showing ZP1 (red) and DAPI (blue) imaging. Scale bar: 20 μm. (E) Effects of ZP3 mutations (p.Asp242Tyr and p.Asn272Ser) on intracellular localization and expression of ZP3 proteins by IF staining. Confocal microscopy image showing images of ZP3 (red), anti-FLAG (green), and DAPI (blue). Scale bar: 25 μm

Back to article page

Journal of Ovarian Research

ISSN: 1757-2215

Contact us