Fig. 4

YJD affected angiogenesis and oxidative stress in POF rats. (A) IHC was performed to detect the expression of CD31 and α-SMA, which is located around growing follicles in the ovaries; Black arrows point to areas of positive staining. (B) Average optical density of CD31. (C) The average optical density of α-SMA. (D–H) Serum levels of the hormones P, E2, FSH, LH, and AMH were examined by ELISA. (I–J) Serum levels of indicators of oxidative stress, MDA and SOD, were assessed by ELISA. ***P < 0.001 vs. Control; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs. TP; ^&P < 0.05, ^&&P < 0.01, ^&&&P < 0.001 vs. TP + M-YJD