Fig. 6

YJD affected the VEGF/VEGFR-2/FAK pathway and KGN cell injury in vitro. (A) Screening of YJD for medicated serum concentrations utilized the CCK-8 assay. (B) The cell viability of each group was measured by CCK-8. (C–G) Serum levels of the hormones P, E2, FSH, LH, and AMH were examined by ELISA. (H) The expression of VEGF, VEGFR-2, and FAK was detected by qRT-PCR. (I-J) The expression of VEGF, VEGFR-2, FAK, p-VEGFR-2 and p-FAK were assessed by WB. (K) Apoptosis index was detected by Annexin V-FITC flow cytometry. (L) The expression of Bcl-2, pro-Caspase-3, Caspase-3 and AIF were assessed by WB. **P < 0.01, ***P < 0.001 vs. Control; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs. TP